PHASE A. Immunization of BALB/c Mice and Serum Screening (approximately 8 weeks)
Eight mice are immunized with antigen(s) provided by customer. Antigen conjugation and purification services are available for an additional charge. At day 35, and later if necessary, mice are test bled and screened for titer by ELISA. If no acceptable response is found during this phase, $1500 will be retained for services up to this point and the remaining balance of deposit will be refunded.
PHASE B. Fusion and Screening of Hybridoma Supernatant (approximately an additional 8-10 weeks)
Upon successful completion of Phase A, selected mice will be euthanized and the spleens harvested. A fusion procedure will be performed and the cells will be distributed into six or eight multiwell plates (96 well) per spleen. Screening will be performed by ELISA using previously supplied antigen. Hybridoma lines from up to ten wells with the highest absorbency by ELISA will be amplified and the supernatants supplied to the investigator. If after the first fusion attempt no acceptable development is observed, a second fusion attempt will be made utilizing previously immunized mice remaining from Phase A. At this point if there is still no response, you the PI or PRF&L has the option to terminate at this point, the charge for completion of Phases A and B will be a total of $5000.
PHASE C. Cloning (approximately an additional 6-8 weeks totaling about 22-26 weeks)
One to five previously developed parental hybridomas will be selected, cloned and then screened by ELISA. Clones with desired titer and specificity will be expanded and cryopreserved. At least five clones will be provided and delivered to the customer depending on the stability of chosen hybridomas.
Total completion cost for phases A, B, & C is $7500 per antigen.
PHASE D. Custom Monoclonal Antibody Production by in vitro methods
Yield of approximately 50 to 100mg per batch
The customer's cell line is amplified using three liter tissue culture polyethylene bags. Monoclonal antibodies are isolated using a protein G column. Available only for IgG producing lines. - $1,450
Other protocols are available and the success with a certain protocol is dependent on the cell line.
Initial Cell Culture Charge (only if lines are supplied to us frozen)
Culture / Expand frozen lines and assess quality prior to production - $375 per line
Amplification and Cryopreservation (10 vials @ 1 - 2 million cells per vial, storage for one year) - $450
each additional line - $250
Cryopreservation (if we developed line) - $100 per line per year
One or first line - $350
Each additional line to be tested at the same time - $150 each
One or first line /antibody - $150
Each additional line to be isotyped at the same time - $75 each
Quantitation of Mouse IgG
One or first sample - $250
Each additional sample at the same time - $75 each
Purification of IgG using Protein G Chromatography
(Protein A is available, please inquire)
Up to 25ml - $325
26 to 50ml - $500
51 to 100ml - $725
$55 per sample